backbone pentr dmd donor vector Search Results


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New England Biolabs gateway pentr4 vector backbone
Gateway Pentr4 Vector Backbone, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher recombinant dna reagent pentr d topo
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Thermo Fisher entry vector pentr
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Addgene inc pentr dmd donor vector
Pentr Dmd Donor Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher pentr d topo vector backbone
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Thermo Fisher pentr
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Thermo Fisher pentr/d-topo
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Qiagen mirneasy plasm/plasma kit
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Vector Laboratories vector2 multilabel counter
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Vector Laboratories substrate vectora blue
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Genechem pcdna3.1-nc
DNMT1 overexpression reversed the impact of PPARα overexpression on apoptosis and ROS levels in HG-treated HRCPs. HRCPs were co-transfected with pCEP4-PPARα, pCEP4-NC <t>and</t> <t>pcDNA3.1-DNMT1</t> or pcDNA3.1-NC, and then treated with 30 mM glucose. QRT-PCR ( A ) and WB ( B , C ) were performed to assess PPARα gene and protein expression in HRCPs. D , E Flow cytometry was performed to estimate apoptosis of HRCPs. F , G The levels of ROS in HRCPs was detected by DCFH-DA fluorescent probe. * P < 0.05 vs. Vector1; # P < 0.05 vs. PPARα + <t>vector2</t>
Pcdna3.1 Nc, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GenScript corporation pglsara vector3
DNMT1 overexpression reversed the impact of PPARα overexpression on apoptosis and ROS levels in HG-treated HRCPs. HRCPs were co-transfected with pCEP4-PPARα, pCEP4-NC <t>and</t> <t>pcDNA3.1-DNMT1</t> or pcDNA3.1-NC, and then treated with 30 mM glucose. QRT-PCR ( A ) and WB ( B , C ) were performed to assess PPARα gene and protein expression in HRCPs. D , E Flow cytometry was performed to estimate apoptosis of HRCPs. F , G The levels of ROS in HRCPs was detected by DCFH-DA fluorescent probe. * P < 0.05 vs. Vector1; # P < 0.05 vs. PPARα + <t>vector2</t>
Pglsara Vector3, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


DNMT1 overexpression reversed the impact of PPARα overexpression on apoptosis and ROS levels in HG-treated HRCPs. HRCPs were co-transfected with pCEP4-PPARα, pCEP4-NC and pcDNA3.1-DNMT1 or pcDNA3.1-NC, and then treated with 30 mM glucose. QRT-PCR ( A ) and WB ( B , C ) were performed to assess PPARα gene and protein expression in HRCPs. D , E Flow cytometry was performed to estimate apoptosis of HRCPs. F , G The levels of ROS in HRCPs was detected by DCFH-DA fluorescent probe. * P < 0.05 vs. Vector1; # P < 0.05 vs. PPARα + vector2

Journal: Biological Research

Article Title: DNMT1-mediated PPARα methylation aggravates damage of retinal tissues in diabetic retinopathy mice

doi: 10.1186/s40659-021-00347-1

Figure Lengend Snippet: DNMT1 overexpression reversed the impact of PPARα overexpression on apoptosis and ROS levels in HG-treated HRCPs. HRCPs were co-transfected with pCEP4-PPARα, pCEP4-NC and pcDNA3.1-DNMT1 or pcDNA3.1-NC, and then treated with 30 mM glucose. QRT-PCR ( A ) and WB ( B , C ) were performed to assess PPARα gene and protein expression in HRCPs. D , E Flow cytometry was performed to estimate apoptosis of HRCPs. F , G The levels of ROS in HRCPs was detected by DCFH-DA fluorescent probe. * P < 0.05 vs. Vector1; # P < 0.05 vs. PPARα + vector2

Article Snippet: The vector pcDNA3.1-DNMT1 overexpressed DNMT1, and the corresponding empty vector pcDNA3.1-NC (vector2) were constructed by GeneChem.

Techniques: Over Expression, Transfection, Quantitative RT-PCR, Expressing, Flow Cytometry